Figure 1.

Representative images of trypanosomatids and co-infection of L. martiniquensis and Crithidia sp. in naturally infected C. peregrinus biting midges. (A) Wing pattern of C. peregrinus. (B) Trypanosomatids in the hindgut of the midge KP22. Red arrowheads indicate choanomastigotes and arrows indicate promastigotes. (C) Leishmania parasites and Crithidia sp. co-infected in the midge KP10. Red arrowheads and black arrowheads indicate trypanosomatids in the foregut and the hindgut, respectively. Red rectangle indicates an area in the foregut showing the movement of Leishmania parasites in Supplementary Video S1. Black rectangle indicates an area in the hindgut showing the movement of Crithidia sp. in Supplementary Video S2. (D) Representative of various forms of Crithidia sp. in the midge KP22. (E–H) Representative promastigote forms of Leishmania parasites in the midge KP19. (E) Procyclic promastigotes, aggregated form. (F) Procyclic promastigote. (G) Nectomonad promastigote. (H) Leptomonad promastigotes.