Table 1.
Natural infection of trypanosomatids in C. peregrinus collected from both locations.
| Location: Tha Ruea | Matched species (Genbank accession no., % best match); [Genbank accession no. of the sequence in this study] | ||||
|---|---|---|---|---|---|
| Insect code | culture | Molecular targets | |||
| ITS1 | 3’UTR-HSP70-I | SSU rRNAe | gGAPDH | ||
| TR1, TR42 | (−) | NMa | NDb | Crithidia sp. (OP698038, 99.46%); Cr. thermophila (KY264937.1, 97.64%); [OR077444- OR077445] | ND |
| TR2 | (+) | NM | ND | Crithidia sp. (OP698038, 99.46%); Cr. thermophila (KY264937.1, 97.63%); [OR077446] | Cr. fasciculata (AF047493.1, 94.41%); [OR088272] |
| TR3 | (+) | NM | ND | Crithidia sp. (OP698038, 99.46%); Cr. thermophila (KY264937.1, 97.64%); [OR077447] | Cr. fasciculata (AF047493.1, 94.60%); [OR088273] |
| TR17c | (−) | L. martiniquensis (MK603827.1, 98.94%); [OR077858] | L. martiniquensis (MK607437.1, 100%); [OR088268] | Crithidia sp. (OP698038, 99.46%); Cr. thermophila (KY264937.1, 97.64%); [OR077448] | ND |
| TR18 | (−) | NM | ND | Crithidia sp. (OP698038, 98.92%); Cr. thermophila (KY264937.1, 97.31%), [OR077449] | ND |
| TR19 | (−) | NM | ND | Crithidia sp. (OP698037, 99.24%); Cr. thermophila (KY264937.1, 97.00%); [OR077450] | ND |
| TR20, TR27, TR29 | (−) | NM | ND | Crithidia sp. (OP217136, 100%); Crithidia sp. (MW694347.1, 99.03%); [OR077451 - OR077453] | ND |
| TR21 | (−) | NM | ND | Crithidia sp. (OP217136, 99.89%); Crithidia sp. (MW694347.1, 98.92%); [OR077454] | ND |
| Location: Khuan Phang | |||||
| KP1 | (+) | NM | ND | Crithidia sp. (OP698038, 99.57%); Cr. thermophila (KY264937.1, 97.64%); [OR077455] | Cr. brachyflagelli (JF717835.1, 94.61%); [OR088274] |
| KP2 | (−) | NM | ND | Crithidia sp. (OP698038, 99.24%); Cr. thermophila (KY264937.1, 97.42%); [OR077456] | ND |
| KP3 | (−) | NM | ND | Crithidia sp. (OP698037, 99.74%); Cr. thermophila (KY264937.1, 97.31%); [OR077457] | ND |
| KP4 | (+) | NM | ND | Crithidia sp. (OP698038, 99.89%); Cr. thermophila (KY264937.1, 97.53%); [OR077458] | Cr. fasciculata (AF047493.1, 94.39%); [OR088275] |
| KP10c | (−) | L. martiniquensis (MK603827.1, 98.94%); [OR077859] | L. martiniquensis (MK607435.1, 98.64%); [OR088269] | Crithidia sp. (OP698038, 99.68%); Cr. thermophila (KY264937.1, 97.74%), [OR077459] | ND |
| KP17d | (−) | L. martiniquensis (MK603827.1, 98.94%); [OR077860] | L. martiniquensis (MK607435.1, 99.77%); [OR088270] | No PCR product | ND |
| KP18, KP28 | (−) | NM | ND | Crithidia sp. (OP698038, 99.68%); Cr. thermophila (KY264937.1, 97.74%); [OR077460 - OR077461] | ND |
| KP19d | (−) | L. martiniquensis (MK603827.1, 98.94%); [OR077861] | L. martiniquensis (MK607437.1, 100%); [OR088271] | No PCR product | ND |
| KP22 | (−) | NM | ND | Crithidia sp. (OP698037, 99.35%); Cr. thermophila (KY264937.1, 97.10%); [OR077462] | ND |
| KP24, KP25, KP30, KP32 | (−) | NM | ND | Crithidia sp. (OP698037, 99.78%); Cr. thermophila (KY264937.1, 97.31%); [OR077463 - OR077466] | ND |
| KP47 | (−) | NM | ND | Crithidia sp. (OP217136, 99.89%); Crithidia sp. (MW694347.1, 99.14%); [OR077467] | ND |
aNM, not matched; bND, not done; cCo-infection of L. martiniquensis and Crithidia sp.; dL. martiniquensis infection; eThe best matched species and the relevant matched species.