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. 2023 Aug 22;14:1222267. doi: 10.3389/fimmu.2023.1222267

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Copyright © 2023 Ferguson, Delgado, McBride, Orbe, La Vake, Caimano, Mendez, Moraes, Schryvers, Moody, Radolf, Weiner and Hawley

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BamA ECL4 is a target antigen for mAb generation. (A) Ribbon diagram for the structural model of Tp BamA (TP0326) depicting the β-barrel, ECL4, and the five periplasmic polypeptide-transport-associated (POTRA) domains. ECLs 4, 6, and 7 form a dome that occludes the barrel opening. (B) Sequences of the three overlapping ECL4 peptides (S1–S3) used for Epivolve. The modified aspartic acid residue in each peptide is represented in black. A light blue line indicates predicted linear B-cell epitopes in S2 and S3. Asterisk indicates an additional residue added to centrally position the modified residue in the S1 peptide. Blue arrow above the sequence indicates the glutamine to leucine substitution in the Mexico A strain of T. pallidum (10). (C) Representative titration ELISAs using native and modified peptides, pre- and post-error-prone PCR.