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. 2023 Sep 4;21:227. doi: 10.1186/s12964-023-01247-5

Fig. 3.

Fig. 3

Construction of anoikis model to construct an anoikis model, different approaches can be used, including the use of specialized cell culture dishes and various drugs. Common drugs that induce apoptosis include H2O2, tunicamycin, thapsigargin, staurosporine, and TNF-α. These drugs can induce anoikis through different mechanisms, such as oxidative stress, endoplasmic reticulum stress, and protein aggregation. Therefore, the selection of these drugs should be based on specific experimental conditions and research purposes