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. 2023 Sep 4;14:232. doi: 10.1186/s13287-023-03469-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Multiple injections of ENSCs to colon of nNOS^−/− mouse increase cell coverage. Immunohistochemical characterization of ENSCs (A) and their transplantation to mouse mid-colon in vivo (B). Six weeks following cell injection, wholemount preparation was used to measure the area covered by transplanted ENSCs (C). Multiple injections covered significantly larger area than single injection (D, *p < 0.05). Scale bars 50 µm (A′–A′″) and 1 mm (B and C)