Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 4;21:336. doi: 10.1186/s12916-023-03034-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — The molecular characteristics of colorectal adenoma are preserved in organoid cultures. A Clustered heatmap of log2-transformed RPKMs showed the 3511 differentially expressed genes in adenomas, CA organoids, and normal mucosae originated from four patients (P1, P2, P4, P5). Stem genes were marked. M, mucosa; A, adenoma; O, organoid. B Gene set enrichment analysis (GSEA) indicated a gene list containing the upregulated genes in adenoma compared with normal mucosa. NES, normalized enrichment score. FDR, false discovery rate. C Immunohistochemical staining displayed the expression of OLFM4 in CA organoid and corresponding primary adenoma and normal mucosa. A representative result of three independent experiments was shown. Scale bar, 100 μm. D Clustered heatmap of log2-transformed RPKMs showed the expression of indicated stem genes between adenoma and normal mucosa. E Adenoma and normal mucosa were harvested to verify expression of c-Myc, LGR5, OLFM4, CD44, CD166– and CD133 using qRT-PCR. All data were presented as mean ± SD (n = 7) and subjected to Student’s t-test in E. *** indicates P < 0.001 versus mucosa. * indicates P < 0.05. ns indicates no significant difference. F Clustered heatmap of normalized RPKMs showed the 2689 differentially expressed genes in CA organoids originated from four patients (P1, P2, P4, P5) and four representative colorectal adenocarcinoma organoids labeled as P17.2, P19b.1, P20.11, and P31.12 from GSE65253. O, CA organoids; C, colorectal adenocarcinoma organoids