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Radiography images and relative quantification of autophosphorylation extent of 3 μM BtNahK titrated by (A) 0–80 μM FeIII-BtNosP and (B) 0–100 μM apo-BtNosP. Dose-dependent autophosphorylation inhibition effect was observed by incubating BtNahK with FeIII-BtNosP but not apo-BtNosP. The plots shown represent an average and the standard deviation of three biological replicates.
