(A) Sequence alignment of the human ETS domains, sorted by the four classes.
(B) Binding motifs of the first listed member in each class as curated in the CIS-BP database.57
(C) One model in the NMR ensemble of the Ets1/DNA complex in which E387 H-bonds with the exocyclic NH2 of two 5′-flanking cytosines. Several other models show only one or the other cytosine being contacted.
(D) DNA-bound PDEF, whose aspartate (D303) does not reach 5′-flanking bases in the 5′-GGAA-3′ strand.
(E) Dipole moments (in debyes) of the four deoxynucleosides, which have been geometry optimized by density functional theory methods at the ωB97X-D/6-311+G(3df,2p) level. The tails of the arrow denote the partial-positive end.