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. Author manuscript; available in PMC: 2023 Sep 5.
Published in final edited form as: Cell Rep. 2023 Jun 22;42(7):112671. doi: 10.1016/j.celrep.2023.112671

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit polyclonal anti-PU.1 Santa Cruz sc-352; RRID:AB_632289
Guinea Pig anti-Rabbit IgG Antibodies Online ABIN101961; RRID:AB_10775589
Bacterial and virus strains
E. coli Stable New England Biolabs C3040
E. coli BL21(DE3)pLyS ThermoFisher C602003
Chemicals, peptides, and recombinant proteins
hPU.1 ETS domain, residue 165 to 270 (ΔN165) Addgene 199796
ΔN165 Q226E This manuscript N/A
Deposited data
CUT&Tag sequencing data for full-length wildtype PU.1 and Q226E in HEK293T cells This manuscript GEO: GSE211518
Co-crystallographic PU.1/DNA structures and electron densities (mmCIF and MTZ) This manuscript For accession numbers, see main text or Table S2
Experimental models: Cell lines
Human: MOLM-13 DMSZ ACC 554
Human: THP-1 ATCC TIB-202; RRID:CVCL_0006
Oligonucleotides
See Table S1 Integrated DNA Technologies N/A
5′-d(GCGAATAA7–deazaGCGGAATGGAAACCG)-3′ Eurogentec N/A
Recombinant DNA
A2-csf1ra-cassette-pLJM2a This manuscript N/A
pMD2.G Didier Trono Addgene 12259
psPAX2 Didier Trono Addgene 12260
pLJM1-EGFP Sancak et al.58 Addgene 19319
FL-hPU.1-wt-pcDNA3.1 Munde et al.69 N/A
FL-hPU.1-Q226E-pcDNA3.1 This manuscript N/A
Software and algorithms
CCP4 CCP4 https://www.ccp4.ac.uk/
Phenix Phenix Online https://phenix-online.org
Coot University of Cambridge https://bernhardcl.github.io/coot/wincoot-download.html
PyMOL Schrödinger https://pymol.org
3DNA Lu et al.64 http://web.x3dna.org/
BANΔIT Barthels et al.65 https://bandit.uni-mainz.de/
OriginPro OriginLab https://www.originlab.com/
Spartan’20 Wavefunction https://www.wavefun.com/products
GROMACS GROMACS https://www.gromacs.org/index.html
FlowJo BD Biosciences https://www.bdbiosciences.com/en-us/products/software/flowjo-v10-software
Picard Tools Broad Institute https://broadinstitute.github.io/picard
Trim Galore! Babraham Institute https://www.bioinformatics.babraham.ac.uk/projects/trim_galore
Homer UCSD http://homer.ucsd.edu/homer
SeqKit Shen et al.72 https://bioinf.shenwei.me/seqkit
REDUCE Roven et al.73 http://reducesuite.bussemakerlab.org/