Antibodies |
Rabbit polyclonal anti-PU.1 |
Santa Cruz |
sc-352; RRID:AB_632289 |
Guinea Pig anti-Rabbit IgG |
Antibodies Online |
ABIN101961; RRID:AB_10775589 |
Bacterial and virus strains |
E. coli Stable |
New England Biolabs |
C3040 |
E. coli BL21(DE3)pLyS |
ThermoFisher |
C602003 |
Chemicals, peptides, and recombinant proteins |
hPU.1 ETS domain, residue 165 to 270 (ΔN165) |
Addgene |
199796 |
ΔN165 Q226E |
This manuscript |
N/A |
Deposited data |
CUT&Tag sequencing data for full-length wildtype PU.1 and Q226E in HEK293T cells |
This manuscript |
GEO: GSE211518
|
Co-crystallographic PU.1/DNA structures and electron densities (mmCIF and MTZ) |
This manuscript |
For accession numbers, see main text or Table S2
|
Experimental models: Cell lines |
Human: MOLM-13 |
DMSZ |
ACC 554 |
Human: THP-1 |
ATCC |
TIB-202; RRID:CVCL_0006 |
Oligonucleotides |
See Table S1
|
Integrated DNA Technologies |
N/A |
5′-d(GCGAATAA7–deazaGCGGAATGGAAACCG)-3′ |
Eurogentec |
N/A |
Recombinant DNA |
A2-csf1ra-cassette-pLJM2a |
This manuscript |
N/A |
pMD2.G |
Didier Trono |
Addgene 12259 |
psPAX2 |
Didier Trono |
Addgene 12260 |
pLJM1-EGFP |
Sancak et al.58
|
Addgene 19319 |
FL-hPU.1-wt-pcDNA3.1 |
Munde et al.69
|
N/A |
FL-hPU.1-Q226E-pcDNA3.1 |
This manuscript |
N/A |
Software and algorithms |
CCP4 |
CCP4 |
https://www.ccp4.ac.uk/
|
Phenix |
Phenix Online |
https://phenix-online.org
|
Coot |
University of Cambridge |
https://bernhardcl.github.io/coot/wincoot-download.html
|
PyMOL |
Schrödinger |
https://pymol.org
|
3DNA |
Lu et al.64
|
http://web.x3dna.org/
|
BANΔIT |
Barthels et al.65
|
https://bandit.uni-mainz.de/
|
OriginPro |
OriginLab |
https://www.originlab.com/
|
Spartan’20 |
Wavefunction |
https://www.wavefun.com/products
|
GROMACS |
GROMACS |
https://www.gromacs.org/index.html
|
FlowJo |
BD Biosciences |
https://www.bdbiosciences.com/en-us/products/software/flowjo-v10-software
|
Picard Tools |
Broad Institute |
https://broadinstitute.github.io/picard
|
Trim Galore! |
Babraham Institute |
https://www.bioinformatics.babraham.ac.uk/projects/trim_galore
|
Homer |
UCSD |
http://homer.ucsd.edu/homer
|
SeqKit |
Shen et al.72
|
https://bioinf.shenwei.me/seqkit
|
REDUCE |
Roven et al.73
|
http://reducesuite.bussemakerlab.org/
|