Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Escherichia coli)	Rosetta 2(DE3) chemically competent cell	Novagen	71402
Strain, strain background (E. coli)	E. coli BL21(DE3) cells	Novagen	69450
Strain, strain background (E. coli)	Trelief 5α chemically competent cell	TSINGKE	TSC-C01-100
Cell line (Homo sapiens)	HEK293T	ATCC	CRL-3216	Authenticated by STR profiling, no mycoplasma contamination
Cell line (H. sapiens)	HeLa	Cell bank of the Chinese Academy of Sciences	SCSP-504	Authenticated by STR profiling, no mycoplasma contamination
Antibody	Anti-GFP rabbit monoclonal antibody	Beyotime	AF1483	WB (1:5000)
Antibody	Anti-RPA32 rabbit monoclonal antibody	Beyotime	AG3115	WB (1:2000), IF (1:50)
Antibody	Anti-β-actin mouse monoclonal antibody	Proteintech	66009–1-Ig	WB (1:5000)
Antibody	Anti-flag tag mouse monoclonal antibody	Beyotime	AF2852	IF (1:50)
Antibody	Horseradish peroxidase labelled goat anti-mouse polyclonal secondary antibody	Beyotime	A0216	WB (1:5000)
Antibody	Horseradish peroxidase labelled goat anti-rabbit polyclonal secondary antibody	Beyotime	A0208	WB (1:5000)
Antibody	Alexa 568 goat anti-rabbit polyclonal antibody IgG (H+L) cross-adsorbed secondary antibody	Thermo Fisher Scientific	A-11036	IF (1:400)
Antibody	Alexa 488 goat anti-mouse polyclonal antibody IgG (H+L) cross-adsorbed secondary antibody	Thermo Fisher Scientific	A32723	IF (1:400)
Recombinant DNA reagent	pcDNA3.1(+)	Invitrogen	V79020
Recombinant DNA reagent	pRSFDuet-1 vector	Novagen	71341
Commercial assay or kit	ClonExpress II One Step Cloning Kit	Vazyme	C112-01/02
Chemical compound, drug	Hydroxyurea	Beyotime	Cat#S1961
Chemical compound, drug	(S)-(+)-Camptothecin, 98%	J&K	Cat#7689-03-4
Chemical compound, drug	2-(4-Amidinophenyl)–6-indolecarbamidine dihydrochloride	Beyotime	C1006	DAPI staining solution used to stain nucleus