Table 1.
Biochemical activity of hybrid compounds bearing a piperazine moiety as a head group
| Compound | PvNMT IC50 (nM)a | HsNMT IC50 (nM)a | SIb |
|---|---|---|---|
| 12a | 36.8 | 5400 | 146.7 |
| 12b | 80.15 | 20,780 | 259.2 |
| 12c | 9.48 | 599 | 63.1 |
| 12d | 48.5 | 7440 | 153.4 |
| 12e | 15.8 | 124 | 7.8 |
| 12f | 15.9 | 699 | 43.9 |
| 16a | 9390 | – | – |
| 16b | 168 | 27,620 | 164.4 |
| 12g | 104 | 15,900 | 152.8 |
| 12h | 440.6 | >40,000 | 90.9 |
| 12i | 1540 | – | – |
| 12j | 124 | 27,360 | 220.6 |
| 12k | 3120 | – | – |
| 12l | 23.4 | 1710 | 73 |
| 16c | >40,000 | – | – |
| 16d | 3390 | – | – |
| 12m | 12,540 | – | – |
| 12n | 604 | 39,730 | 65.7 |
| 12o | 3116 | – | - |
| 12p | 368 | 23,080 | 62.7 |
| 12q | 73 | 1632 | 22 |
| 16e | 5070 | – | – |
| 16f | 14,033 | – | – |
aPvNMT and HsNMT IC50 values are shown as mean values of two or more determinations.
bEnzyme selectivity calculated as HsNMT IC50/PvNMT IC50.