Fig. 7. TMAO showed no capacity in inducing folding of C71G-hPFN1 and hSOD1.
a Up-field 1D and HSQC spectra of C71G-hPFN1 at a concentration of 50 μM in the presence of TMAO at molar ratios up to 1:2000 (C71G:TMAO) at which TMAO triggered a complete precipitation. For clarity, only 1D spectra of C71G-hPFN1 in the free state (black) and in the presence of TMAO at 1:2000 were presented. b Up-field 1D and HSQC spectra of hSOD1 at a concentration of 50 μM in the presence of TMAO at molar ratios up to 1:1000 (hSOD1:TMAO) at which TMAO triggered a complete precipitation.