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. 2023 Aug 15;26(9):107646. doi: 10.1016/j.isci.2023.107646

Figure 6.

Figure 6

AT9283 and dorsomorphin inhibits the receptor tyrosine kinase TEK

HUVECs were treated with Ang1 (200 ng/mL) in the presence or absence of AT9283 (or Dorsomorphin) for 24 h.

(A–B) The inhibition of TEK kinase activity by AT9283 and dorsomorphin was measured by an ELISA kit for human phospho-TEK. n = 6 in each group.

(C–E) DM, Dorsomorphin (C–E) Representative western blot images and protein quantification results showing changes in the protein phosphorylation levels of TEK and the downstream protein Akt after dorsomorphin treatment. β-Actin was used as loading control. n = 5 in each group. ∗p < 0.05 vs. control, #p < 0.05 vs. Ang1. Data are shown as mean ± SEM. DM, Dorsomorphin.

(F–H) Representative western blot images and protein quantification results showing changes in the protein phosphorylation levels of TEK and the downstream protein Akt after AT9283 treatment. β-Actin was used as loading control. n = 5 in each group. ∗p < 0.05 vs. control, #p < 0.05 vs. Ang1. Data are shown as mean ± SEM.