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. 2023 Sep 5;14:5411. doi: 10.1038/s41467-023-40993-5

Fig. 1. IL-33 deficiency increases the severity of intestinal radiation injury and impairs epithelial regeneration.

Fig. 1

a Time course of IL-33 protein in small intestine (SI) at baseline and over 5 days after 10 Gy TBI (n = 7 mice/group), measured by ELISA and normalized to mg of total protein; data combined from two experiments. b Relative expression of soluble IL-33 receptor (sST2) measured by qPCR in SI crypts from WT and IL-33 KO mice following 10 Gy TBI (n = 9 WT and n = 6 IL-33 KO mice/group); data combined from three independent experiments. c–e Terminal SI histology (ileum) in WT and IL-33 KO mice at baseline and 5 days after TBI; shown are representative images (c) and quantified analyses of crypt number (d) and size (e) combined from two experiments (n = 6 mice/group). f, g Representative images (f) and quantification (g) of Ki67 immunohistochemistry in ileal crypts at baseline and five days after TBI (10 Gy); data combined from two experiments (n = 5 mice/group). h, i Representative immunofluorescent images (h) and quantification (i) of Lgr5-LacZ+ cells stained for anti-β-galactosidase (red) and nuclei (blue) in ileum on day 5 after TBI (10 Gy); data combined from two experiments (n = 5 mice/group). Statistical analyses were performed using one-way ANOVA multiple comparison testing (a, d, e, g, i), or two-tailed Mann-Whitney U (b). All dot plots (a, b, d, e, g, i) show means, and error bars indicate SEM (a, b); *p < 0.05, **p < 0.01, ***p < 0.001; scale bars: 100 µm. Source data for graphs are provided in the Source Data file. The exact p-values are as follows: (a) comparisons made vs. day 0; day 1, p = 0.1014; day 2, p = 0.0013; day 3, p = 0.0002; day 4, p = 0.0972; day 5 p = 0.9851; (b) WT vs KO comparisons made for each timepoint; day 0, p = 0.8374; day 1, p = 0.0176; day 2, p = 0.0004; day 3, p = 0.0004; day 4, p = 0.9792; day 5, p = 0.7544; (d) unirradiated WT vs unirradiated KO, p = 0.37; unirradiated WT vs 10 Gy WT, p = 0.0008; unirradiated KO vs 10 Gy KO, p = < 0.0001; 10 Gy WT vs 10 Gy KO, p = 0.0343. e unirradiated WT vs unirradiated KO, p = 0.99; unirradiated WT vs 10 Gy WT, p = 0.0007; unirradiated KO vs 10 Gy KO, p = 0.67; 10 Gy WT vs 10 Gy KO, p = 0.007; (g) unirradiated WT vs unirradiated KO, p = 0.94; unirradiated WT vs 10 Gy WT, p = < 0.0001; unirradiated KO vs 10 Gy KO, p = 0.11; 10 Gy WT vs 10 Gy KO, p = 0.0084; (i) unirradiated WT vs unirradiated KO, p = 0.99; 10 Gy WT vs 10 Gy KO 10 Gy p = 0.039.