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. 2023 Sep 5;14:5415. doi: 10.1038/s41467-023-41218-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Coimmunoprecipitation and immunoassay for extracts of HEK293T cells transfected with HA-AhR (WT), MYC-STING (WT), STING (1-139), and STING (140–379) by anti-MYC beads. B Coimmunoprecipitation and immunoassay for extracts of HEK293T cells transfected with MYC-AhR (WT), AhR (1-120), AhR (120–424), AhR (1–424), AhR (424–848), and HA-STING (WT) by anti-MYC beads. C Coimmunoprecipitation and immunoassay for extracts of HEK293T cells transfected with MYC-AhR (WT), AhR (1–277;424–848), and HA-STING (WT) by anti-MYC beads. D The RMSD values for AhR-STING, AhR-STING-KYN, AhR-STING (dimer), and AhR-STING-KYN (dimer) of the simulation. E–L The simulation of the interaction and surface binding model between AhR and STING, including AHR-STING (E, F), AhR-STING-KYN (G, H), AhR-STING (dimer) (I, J), and AhR-STING-KYN (dimer) (K, L). AhR is colored with marine, STING with magenta, KYN with yellow. The key residues in AhR are shown as cyan sticks while others as marine stick. The key residues in STING are shown as cyan sticks while others as magenta stick. The red dashes represent hydrogen bond interaction. The blue dashes represent the salt bridge. M, N Coimmunoprecipitation and immunoassay for extracts of HEK293T cells transfected with MYC-STING (140–379), FLAG-AhR (WT) along with HA-UB or its mutants by anti-MYC beads. O, P Coimmunoprecipitation and immunoassay for extracts of cell lysate of AhR knocked-out cells by anti-STING antibodies. Q, R MYC-STING (140–379) or its mutants were individually transfected into HEK293T cells along with HA-AhR by anti-MYC beads. K0 denotes STING with all lysine residues mutated to arginine. S Immunoassay for STING expression of K236R cells after indicated treatment in different time points (Mean ± SD). P value < 0.05 as statistic difference by two-tailed t test. T Immunoassay for STING expression of K236R cells after indicated treatment. U Coimmunoprecipitation and immunoassay for extracts of K236R cells lysate after treated with Kyn by anti-STING antibodies. V Coimmunoprecipitation and immunoassay for extracts of HEK293T cells transfected with MYC-STING (WT), MYC-STING (K236R), and FLAG-TBK1 (WT) by anti-MYC beads after treated with cGAMP. Three biologically independent experiments were performed (A–C, M–V). Source data are provided as a Source Data file.