Figure 2.

NORAD KD effects on tumor-relevant phenotypes

(A) NORAD levels in the MDA-MB-468 cell line (smRNA FISH) treated with control siRNA + LNA or NORAD-specific siRNA + LNA. (B and C) NORAD KD effect on cell migration in the MDA-MB-231 cell line (wound healing assay); (B) is the gap quantification at the indicated time points (n = 3), (C) is a representative image of the wound healing. (D) NORAD KD sensitizes cells to doxorubicin (DXR), measured through the alamarBlue reduction assay (n = 3). (E and F) NORAD KD and doxorubicin effects on cell apoptosis in the MDA-MB-231 cell line (n = 3) as measured through the increase in Annexin V⁺ cells and as visualized in the representative plots (F). No symbol, p > 0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.