Table 2.
Antibody | Type | Reference | Dilution |
---|---|---|---|
γH2AX | primary antibody | ab2893 (Abcam) | 1:1,000 |
PUM2 | primary antibody | ab92390 (Abcam) | 1:10,000 |
H2AX | primary antibody | 10856-1-AP (Proteintech) | 1:2,000 |
PARP1 | primary antibody | MA3-950 (Thermo Fisher Scientific) | 1:500 |
CDK1 | primary antibody | Ab131450 (Abcam) | 1:500 |
Anti-mouse | cross-adsorbed secondary antibody, HRP | G21040 (Invitrogen) | 1:10,000 |
Anti-rabbit | cross-adsorbed secondary antibody, HRP | G21234 (Invitrogen) | 1:10,000 |