Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 5;6:911. doi: 10.1038/s42003-023-05269-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Diagrams of INPP5E truncations. Proline-rich domain (PRD), inositol polyphosphate phosphatase catalytic (IPPc) domain, CaaX motif are marked in yellow, blue, and purple, respectively. The CTS indicates the ciliary targeting sequence. b Jurkat cells were transfected with different truncations of Flag-INPP5E. The expression levels of Flag-INPP5E truncations were examined by western blots. α-tubulin was used as the loading control. c Immunostaining of Flag-INPP5E truncations in conjugates of Jurkat T cells and CMAC-labeled SEE-pulsed APCs. Cells were co-stained with anti-CD3ε as an immune synapse marker. Scale bar: 10 μm. d Quantification of the Flag-INPP5E recruitment index (upper) and the percentage of conjugates with Flag-INPP5E truncations at the immune synapse (lower) is shown. The recruitment of Flag-INPP5E truncations to the immune synapse was shown by the scatter plot graph. 30–50 conjugates were quantified for each INPP5E mutant. Images are representative of two experiments. Error bars indicate mean ± SD. One-way ANOVA analysis. *P ≤ 0.05. **P ≤ 0.005. ****P < 0.0001. e Summary of INPP5E truncations in the localization of primary cilia and immune synapse (IS).