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. 2023 Aug 30;95:104778. doi: 10.1016/j.ebiom.2023.104778

Fig. 4.

Fig. 4

Exposure to LC serum recapitulates some of the Treg functional abnormalities observed in LC patients. a.-e. Healthy CD4+CD25+ Tregs were incubated with 25% HC or LC Serum for 24 h. a. FoxP3 expression (MFI). HC/LC serum n = 5. Unpaired t-test following log10 transformation. Median (IQR). b. Percentage of Annexin V+ Tregs. HC/LC serum n = 7. Unpaired t-test following log10 transformation. Median (IQR). c. Proportion of IL-17 (left) and IFNγ (right) expressing Tregs. HC/LC serum n = 5. Multiple unpaired t-tests with Bonferroni adjustment following log10 transformation. Median (IQR). d. Suppressive capacity of healthy Tregs after pre-incubating in 25% serum of HC or LC patients for 24 h. HC/LC serum n = 9. Two-way repeated measures ANOVA with Bonferroni's correction for multiple pairwise comparison. Values shown as SD. e. Metabolic profiling using Seahorse of CD4+CD25+ Tregs following incubation in 25% HC and LC Serum together with anti-CD3/CD28 beads (1:5 = bead to cell ratio) for 24 h. Oxygen Consumption Rate (OCR) and extracellular acidification rate (ECAR) was measured following the exposure to Oligomycin (Oligo), FCCP and Rotenone + Antimycin A (R + A). f. Left: basal and maximal OCR; and ATP production (measured after Oligomycin treatment) in HC and LC Tregs. Right: basal ECAR, glycolytic capacity (max. ECAR) and basal OCR/ECAR ratio in HC and LC Tregs. HC n = 9, LC n = 6. Unpaired t-tests following log10 transformation. Median (IQR). Abbreviations: Healthy control (HC), liver cirrhosis (LC), Oxygen consumption rate (OCR), Extracellular acidification rate (ECAR), Oligomycin (Oligo), Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP), Rotenone + Antimycin A (R + A).