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. 2023 Aug 19;26(9):107690. doi: 10.1016/j.isci.2023.107690

Figure 2.

Figure 2

Infected sensory neurons synthesize SARS-CoV-2 RNA but do not replicate progeny virion

(A) Fraction of negative strand SARS-CoV-2 RNA compared to the total number of SARS-CoV-2 RNA. Negative strand SARS-CoV-2 RNA was detected in sensory neurons and 293T cells positive for SARS-CoV-2 RNA in scRNA-seq.

(B) Plaque forming assay on hiPSC-derived cortical and sensory neurons and Calu-3 cells exposed to WA1/2020 SARS-CoV-2 for 24, 48 and 72 h. N = 3; t-test ∗p value<0.05. Mean ± S.E.M.

(C) Detection of viral dsRNA and neuronal marker beta3-Tubulin (TUBB3) by immunofluorescence in hiPSC-sensory neurons co-cultured with HEK293T cells exposed to WA1/2020 SARS-CoV-2 for 48h. Scale: 20μm. C′ zoom of the inset presented in C.

(D) Detection of SARS-CoV-2’s Nucleocapsid and neuronal marker beta3-Tubulin (TUBB3) by immunofluorescence in hiPSC-sensory neurons co-cultured with HEK293T cells exposed to WA1/2020 SARS-CoV-2 for 48h. Scale: 20μm. D′ zoom of the inset presented in D.

(E) Boxplot of average Nucleocapsid staining intensity in the axons of hiPSC-sensory neurons co-cultured with HEK293T cells exposed to WA1/2020 SARS-CoV-2 for 48h. Average with min and max, +/− SD.