Fig. 9.

CD2/CD27 could influence M2 polarization of macrophages and participate in breast cancer brain metastasis.

Note: (A) IVIS spectral system is used for in vivo bioluminescence imaging to monitor tumor growth; (B) H&E staining is used to observe the morphology of nude mouse tumor tissue (× 200); (C) TUNEL staining is used to detect cell apoptosis in nude mouse tumor tissue (× 200); (D) Ki67 immunohistochemical staining is used to detect Ki67 protein expression in nude mouse tumor tissue (× 200); (E) RT-qPCR is used to detect the expression of CD2 and CD27 mRNA in nude mouse tumor tissue; (F) WB is used to detect the expression of CD2 and CD27 protein in nude mouse tumor tissue; (G) Flow cytometry is used to detect the proportion of macrophages in each group of nude mouse tumor tissue. (H) The Glutamine content in tumor tissues of each group was measured using the Glutamine Assay Kit. * indicates P<0.05 compared with the oe-NC group, with 8 nude mice in each group.