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. 2023 Aug 23;26(9):107705. doi: 10.1016/j.isci.2023.107705

Figure 4.

Figure 4

SARS-CoV-2 Nsp15 interacts with TBK1

(A) The plasmids of Flag-tagged RIG-I, MAVS, IRF3, TBK1, NuTF2, RNF41 and HA-Nsp15 were co-transfected into HEK-293T cells, and 24 h later, co-immunoprecipitation was performed using an anti-Flag Affinity Gel.

(B and C) Purified GST-Nsp15 protein was incubated with Flag-TBK1 overexpressed or un-transfected HEK-293T cell lysates, and GST-Nsp15 interaction with Flag-TBK1 (B) or endogenous TBK1 (C) was analyzed by Western blot.

(D) HeLa cells were transfected with the HA-Nsp15 and Flag-TBK1 expression plasmids for 24 h. Cells were immobilized and immunostained for detection of Nsp15 (Anti-HA, green) and TBK1 (Anti-Flag, red), and the nucleus was stained with DAPI (blue). Scale bar, 25 μm. All experiments were performed at least three times.