REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IRF-3 (D6I4C) XP® Rabbit mAb	Cell Signaling Technology	Cat#11904S; RRID: AB_2722521
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb	Cell Signaling Technology	Cat#4947S; RRID: AB_823547
TBK1/NAK (E8I3G) Rabbit mAb	Cell Signaling Technology	Cat#38066S; RRID: AB_2827657
Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb	Cell Signaling Technology	Cat#5483S; RRID: AB_10693472
DYKDDDDK Tag (D6W5B) Rabbit mAb	Cell Signaling Technology	Cat#14793S; RRID: AB_2572291
HA tag Rabbit Monoclonal Antibody	Cell Signaling Technology	Cat#5017; RRID: AB_10693385
GST Mouse Monoclonal Antibody	Sigma-Aldrich	Cat#G1160; RRID: AB_259845
Myc Tag Mouse Monoclonal Antibody	Beyotime	Cat#AF0033; RRID: AB_2939055
GFP Rabbit Monoclonal Antibody	Cell Signaling Technology	Cat#2956; RRID: AB_1196615
Alexa Fluor 488-labeled Goat Anti-Rabbit IgG(H + L)	Beyotime	Cat#A0423; RRID: AB_2891323
Alexa Fluor 555-labeled Donkey Anti-Mouse IgG(H + L)	Beyotime	Cat#A0460; RRID: AB_2890133
GAPDH Rabbit mAb	ABclonal	Cat#A19056; RRID: AB_2862549
β-Tubulin Rabbit mAb	ABclonal	Cat#A12289; RRID: AB_2861647
HRP Rabbit Anti-Goat IgG (H + L)	ABclonal	Cat#AS029; RRID: AB_2769859
HRP Goat Anti-Mouse IgG (H + L)	ABclonal	Cat#AS003; RRID: AB_2769851
Bacterial and virus strains
Sendai virus	This paper	N/A
recombinant VSV-GFP strains	This paper	N/A
Chemicals, peptides, and recombinant proteins
Lipo6000™ Transfection Reagent	Beyotime	Cat#C0526;
PMSF	Beyotime	Cat#ST507;
RIPA Lysis Buffer	Beyotime	Cat#P0013D
5×SDS-PAGE Protein Loading Buffer	YEASEN	Cat#20315ES05
GoldBand Plus 3-color Regular Range Protein Marker(8–180 kDa)	YEASEN	Cat#20350ES72
GSTSep Glutathione Agarose Resin	YEASEN	Cat#20507ES10;
InStab™ Phosphatase Inhibitor Cocktail	YEASEN	Cat#20109ES05;
Anti-Flag Affinity Gel	Bimake	Cat#B23101;
Protease Inhibitor Cocktail	Merck	Cat#P8340;
Critical commercial assays
RevertAid First Strand cDNA Synthesis Kit	ThermoFisher Scientific	Cat#K1622;
Dual Luciferase Reporter Assay Kit	Vazyme	Cat#DL101-01;
RNA-easy Isolation Reagent	Vazyme	Cat#R701-01;
ChamQ Universal SYBR qPCR Master Mix	Vazyme	Cat#Q711-02;
Experimental models: Cell lines
HEK-293T	ATCC	N/A
HeLa	ATCC	N/A
Oligonucleotides
Primers for sequences, see Table S1	This paper	N/A
Recombinant DNA
pcDNA3.0-HA	This paper	N/A
pcDNA3.0-Flag	This paper	N/A
pEGFP-C1	This paper	N/A
pET-28a+	This paper	N/A
pGL3-IFN-β-Luc	This paper	N/A
pRL-TK	This paper	N/A
Software and algorithms
ImageJ	National Institutes of Health	Version 1.8.0
GraphPad Prism	GraphPad Software	Version 7.04
LAS AF Lite	Leica microsystems	Version 2.3.5
Geneious Prime	Biomatters	Version 2020.05