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. 2023 Jul 22;26(9):107455. doi: 10.1016/j.isci.2023.107455

Figure 2.

Figure 2

Sympathoexcitation impaired type H vessels and resulted in osteopenia

(A and B) Micro-CT analysis and 3D views of femurs of mice treated with PBS and ISO. ISO was injected intraperitoneally for 4 weeks. Scale bars, 500 μm (A) and 250 μm (B).

(C–F) Parameter analysis of trabecular bone mass. Tb.BV/TV, trabecular bone volume over tissue volume; Tb.Th, trabecular thickness; Tb.Sp, trabecular separation; Tb.N, trabecular number. N = 4 per group.

(G and H) Parameter analysis of cortical bone mass. Ct.Ar, cortical bone area; Ct.Th, cortical bone thickness. N = 4 per group.

(I) CD31 (green) and EMCN (red) co-immunostaining at metaphysis of mice treated with PBS and ISO, counterstained by DAPI (blue). Scale bar, 100 μm.

(J) Quantification of type H vessel density (%). N = 3 per group.

Data represent mean ± SD. Statistical analysis is performed by Student’s t test. ∗∗, p < 0.01; ∗∗∗, p < 0.001.