Fig. 3.

DUXAP10 promotes GC cells growth and cell cycle progression. a MTT assays were used to determine the cell viability for si-DUXAP10 or si-NC transfected BGC823, SGC7901 and MGC803 cells, and DUXAP10 vector or empty vector transfected AGS cells. Values represented the mean ± s.d. from three independent experiments. b Edu staining analysis showing significant decrease of cell viability in si-DUXAP10 transfected BGC823, SGC7901 and MGC803 cells. c Colon formation assays showing significant decrease of cloning viability in si-DUXAP10 transfected GC cells. d FACS analysis shows significant increases or decreases of cells in G1or S phase, respectively, in si-DUXAP10 transfected GC cells. e Cyclin D1, Cyclin D3, CDK2, CDK4, and CDK6 protein levels were detected by western blot analysis after DUXAP10 knockdown. *P < 0.05, **P < 0.01