Table 1.
The virus-related mouse models
| Author | Mouse model type | Mouse strains | Method |
|---|---|---|---|
| Huang et al. [22] | Live tumors |
BALB/c, C57BL/6, FVB and ICR |
AAV/5′-HBV-SD、AAV/3′-HBV-SA, AAV/5-HBV-SD, AAV/3-HBV-SA (AAV/HBV) were intravenously injected into mice at 6–8 weeks. Macroscopic liver tumors appeared between 12 and 16 months after AAV/HBV trans induction. |
| Wu et al. [23] | HCC |
HBs-Tg, (Fah−/−), C57BL6/J, |
Liver parenchymal cells from HBS TG mice were transfused into NTBC-treated FAH deficient mice with healthy immune system at 8–12 weeks of age via spleen injection. HBs-HepR mice with liver replacement reconstruction exhibited chronic hepatitis, liver fibrosis, and liver cancer. |
| Hao et al. [24] | HBs-HepR mice | C57BL/6J | Hepatocytes from HBS-TG mice were transfused into NTBC-treated FAH deficient mice with healthy immune system at 8–10 weeks of age via spleen injection. HBs-HepR mice with liver replacement reconstruction exhibited HCC at 9 months after reconstruction. |
| Chung et al. [26] | TG mice expressing HBp or the RT domain of HBp | C57BL/6 N | The 5.3-kb DNA containing AAP-HBp or the 3.9-kb DNA containing AAP-RT was microinjected into the pronuclei of fertilized mouse eggs. |
| Kim et al. [27] | Transgenic mice harboring entire HBx gene | CD1 | The HBx transgenic mice were derived by microinjection of a 1.15 kb HBV subtype adr DNA fragment into single-cell embryos of CD1 mice. |
| Labonté P et al. [29] | Transplantation model of HCC | CD1 | HuT7-3 cells were injected directly into the liver of nude mouse. |
| Islam et al. [32] | TG mice expressing HCV RNA | C57BL/6 | HCV JFH-1 DNA was injected into a male pronucleus of fertilized eggs. |
| Ernst et al. [33] | HCV TG mouse | C57BL/6x | HCV core-transgenic mice were obtained by pronuclear injection of a purified 4.9 kb AsnI–XmnI fragment of pBI-L core containing the bidirectional expression unit into F1-zygotes (C57Bl/6 X DBA (H2b)). Similarly, the HCV ORF mice were generated by transferring the 13.5 kb fragment of pBI-L ORF into fertilized eggs. |
| Jeannot et al. [36] | Chemically-induced mouse models | C57BL/6 | Neonatal mice were administered a single dose of Aflatoxin B1 (6 µg/g body weight) or tricaprylin vehicle (15 µl/g body weight) by intraperitoneal injection. |
Note: HCC, hepatocellular carcinoma; TG, transgene; HBV, hepatitis B virus; HCV, hepatitis C virus; HBp, HBV polymerase