. 2023 Sep 5;18:49. doi: 10.1186/s13027-023-00524-9

Table 3.

The mouse models of chemically-induced hepatocellular carcinomas

Author	Mouse Model Type	Mouse strains	Method
Vesselinovitch et al. [67]	Chemically-induced mouse models	C57BL/6J and C3HeB/FeJ F₁	C57BL/6J female mice was bred with C3HeB/FeJ F1 male mice to generate C57BL/6J × C3HeB/FeJ F1 mice model(B6C3F1). A single intraperitoneal injection of DEN was administered to 15-day-old B6C3F1 male mice. The B6C3F1 mice were divided into two groups. The first groups of mice were given 0.625, 1.25, 2.5, and 5.0 µg of DEN per g of body weight. Another group of mice was given 0.312, 0.625, 1.25, 2.5, and 5.0 µg of DEN per g of body weight. Under a low dose of nontoxic concentration (0.312 to 5.0 ug/g body weight) of DEN, infant male B6C3F1 mice were successfully induced to develop HCC after an average of 44 weeks.
Leenders et al. [70]	Chemically-induced mouse models	C57BL/6J and DBA/2J	Seven-day-old mice were injected with 6 µg/g body weight of AFB1. By 52 weeks of age, HCC was detected in 90% of DBA/2J male mice while only 27% of C57BL/6J male mice developed HCC.
Uehara T et al. [75]	Chemically-induced mouse models	B6C3F₁	Male mice are administered a single intraperitoneal injection of 1 mg/kg DEN (Dissolve 1 mg DEN in 15 mL PBS on the day of dosing) at 14 days of age. Beginning at 8 weeks of age the animals are intraperitoneally administered 0.2 ml/kg CCl₄ (Dissolve 1 ml CCl₄ in olive oil at the final volume of 10 ml) two times per week for up to 14 weeks.

Author

Mouse Model Type

Mouse strains

Method

Vesselinovitch et al. [67]

Chemically-induced mouse models

C57BL/6J and

C3HeB/FeJ F₁

C57BL/6J female mice was bred with C3HeB/FeJ F1 male mice to generate C57BL/6J × C3HeB/FeJ F1 mice model(B6C3F1). A single intraperitoneal injection of DEN was administered to 15-day-old B6C3F1 male mice. The B6C3F1 mice were divided into two groups. The first groups of mice were given 0.625, 1.25, 2.5, and 5.0 µg of DEN per g of body weight. Another group of mice was given 0.312, 0.625, 1.25, 2.5, and 5.0 µg of DEN per g of body weight. Under a low dose of nontoxic concentration (0.312 to 5.0 ug/g body weight) of DEN, infant male B6C3F1 mice were successfully induced to develop HCC after an average of 44 weeks.

Leenders et al. [70]

Chemically-induced mouse models

C57BL/6J and DBA/2J

Seven-day-old mice were injected with 6 µg/g body weight of AFB1. By 52 weeks of age, HCC was detected in 90% of DBA/2J male mice while only 27% of C57BL/6J male mice developed HCC.

Uehara T et al. [75]

Chemically-induced mouse models

B6C3F₁

Male mice are administered a single intraperitoneal injection of 1 mg/kg DEN (Dissolve 1 mg DEN in 15 mL PBS on the day of dosing) at 14 days of age. Beginning at 8 weeks of age the animals are intraperitoneally administered 0.2 ml/kg CCl₄ (Dissolve 1 ml CCl₄ in olive oil at the final volume of 10 ml) two times per week for up to 14 weeks.

Note: DEN, diethylnitrosamine; AFB1, Aflatoxin B1