. 2023 Sep 5;18:49. doi: 10.1186/s13027-023-00524-9

Table 7.

The mouse models of other liver diseases

Author	Mouse model type	Mouse strains	Method
Santhekadur et al. [119] Tsuchida et al. [121]	NAFLD	B6/129, C57Bl6/J and S129S1/svlmJ mice	Mice (8–12 weeks old) were fed ad libitum a high-fat diet, high carbohydrate diet with 42% kcal from fat and containing 0.1% cholesterol with a high fructose-glucose solution (23.1 g/L d-fructose + 18.9 g/L d-glucose). Obesity, liver injury, dyslipidemia, and insulin resistance were observed between 32 and 52 weeks.
Constandinou et al. [122]	Liver fibrosis	No special instructions	Mice were intraperitoneally injected with a 1:7 (volume/volume) mixture of CCL₄: olive oil every 5 days (a total of 0.125 mL/g CCL₄ per injection) for 4 weeks to induce established fibrosis, and harvested 3 days after the final injection.
Domenicali et al. [124]	Liver fibrosis	C57BL/6NCrl mice	Mice with a body weight between 20 and 25 g were pretreated with phenobarbital (0.3 g/L) dissolved in drinking water. Twice a week, 2 milliliters of a 50% (v/v) CCL₄ solution, containing 1.0 mL per kg of body weight of CCL₄ dissolved in liquid paraffin oil, was injected subcutaneously on the back.

Author

Mouse model type

Mouse strains

Method

Santhekadur et al. [119]

Tsuchida et al. [121]

NAFLD

B6/129, C57Bl6/J and S129S1/svlmJ mice

Mice (8–12 weeks old) were fed ad libitum a high-fat diet, high carbohydrate diet with 42% kcal from fat and containing 0.1% cholesterol with a high fructose-glucose solution (23.1 g/L d-fructose + 18.9 g/L d-glucose). Obesity, liver injury, dyslipidemia, and insulin resistance were observed between 32 and 52 weeks.

Constandinou et al. [122]

Liver fibrosis

No special instructions

Mice were intraperitoneally injected with a 1:7 (volume/volume) mixture of CCL₄: olive oil every 5 days (a total of 0.125 mL/g CCL₄ per injection) for 4 weeks to induce established fibrosis, and harvested 3 days after the final injection.

Domenicali et al. [124]

Liver fibrosis

C57BL/6NCrl mice

Mice with a body weight between 20 and 25 g were pretreated with phenobarbital (0.3 g/L) dissolved in drinking water. Twice a week, 2 milliliters of a 50% (v/v) CCL₄ solution, containing 1.0 mL per kg of body weight of CCL₄ dissolved in liquid paraffin oil, was injected subcutaneously on the back.

Note: NAFLD, nonalcoholic fatty Liver disease