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. 2023 Jul 10;24(9):e56150. doi: 10.15252/embr.202256150

Figure EV3. Pol II accumulation is reduced at promoters in CTD‐∆5 context and lost at 3′ end of coding genes.

Figure EV3

  1. qPCR showing the enrichment of Pol‐HA ChIP on positive regions compared with negative control in rWT (black) and CTD‐∆5 (orange). Left: Percentage of IP over Input for each target. Right: Fold enrichment normalized on negative control. Error bars represent the mean with SD of 3 technical replicates.
  2. Example of Pol‐HA ChIP‐seq signal in rWT (black) and CTD‐∆5 (orange) as well as their associated Input profiles and ChrRNA signals. Light gray rectangle highlights a region in which there is a global accumulation of Pol‐HA in CTD‐∆5 associated with read‐through at ChrRNA level. Right: Extended view of the region highlighted in light gray. Black arrows show promoter‐associated Pol‐HA accumulation in both rWT and CTD‐∆5. Red arrow shows 3′‐end Pol‐HA accumulation in rWT, which is lost in CTD‐∆5 mutant. Experiments were done in biological duplicate, however, only the sample with the best signal‐to‐noise ratio was used for subsequent analysis.