Figure EV4. Polyadenylated transcripts are less affected by CTD‐∆5 mutation.

1. Wide view example of ChrRNA‐seq and its associated polyA signal in rWT and CTD‐∆5.
2. Average metagene profiles of polyA RNA‐seq signals in sense and antisense directions (top and bottom, respectively) over the gene bodies of expressed protein‐coding genes and the 20 kb upstream and downstream surrounding regions in rWT (dotted gray line) and CTD‐∆5 (blue). All profiles are asinh transformed and non‐normalized over gene bodies. Experiments were done in biological duplicates. P‐values associated with read‐through were calculated using two‐sided Wilcoxon tests: 4e‐53 in sense and 9e‐4 in antisense.