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. 2023 Jul 25;24(9):e56230. doi: 10.15252/embr.202256230

Figure EV3. FBL interacts with YBX1 independent of DNA or RNA and its methyltransferase activity.

Figure EV3

  1. To establish cell lines stably expressing epitope‐tagged proteins, HCT116 cells were transfected with plasmids encoding SFB‐FBL. Protein expressions were examined by western blotting with indicated antibodies.
  2. The interaction between FBL and YBX1 is not mediated by DNA or RNA. The association between FBL and YBX1 was examined by coimmunoprecipitation assays in HCT116 cells with RNase A or benzonase treatment.
  3. The catalytically inactive mutant of FBL also binds YBX1 in vivo. Co‐IP analyses of GFP‐FBLE191A/D236A and SFB‐YBX1 in HCT116 cells.