Reagent/Resource	Reference or source	Identifier or catalog number
Experimental models
CEN.PK (S. cerevisiae)	PMID: 16254148
MSP247 (S. cerevisiae)	This study
MSP248 (S. cerevisiae)	This study
X2180‐5A (S. cerevisiae)	PMID: 3519363
Recombinant DNA
pFA6a‐kanMX6	PMID: 9717241
Antibodies
Anti‐Pgk1p antibody, mouse monoclonal	ThermoFisher	459250
Anti‐Rps6 p antibody	Cell Signaling	4858
Goat Anti Mouse HRP antibody	ThermoFisher	31431
Goat Anti Rabbit HRP antibody	ThermoFisher	31466
Anti‐Sch9‐pT737, rabbit polyclonal	PMID: 24514902
Oligonucleotides and sequence‐based reagents
BAT1‐F1 (ACTACATGTTTTCGTTAGAATAAATCACCCTATAAACGCAAAATCAGCTAGAACCTTAGCATACTAAAACCGGATCCCCGGGTTAATTAA)	This study
BAT1‐R1 (AACAGATCCTCTGAGAGGAATTCTCGTTTTTTTTTTTTGGGGGGGGAGGGGATGTTTACCTTCATTATCAGAATTCGAGCTCGTTTAAAC)	This study
BAT2‐F1 (ATAAAAACAGACTAACTACTAAAATTTTAGAAATTTAAGGGAAAGCATCTCCACGAGTTTTAAGAACGATCGGATCCCCGGGTTAATTAA)	This study
BAT2‐R1 (CTCAGTAGAAAAAACGCTCTAGTTTTATTCTTTTTAACTTTTAATTACTTTACGTAGCAATAGCGATACTGAATTCGAGCTCGTTTAAAC)	This study
VIII:517387‐FWD (GGTACCGGCCTTTAAGCTTT)	This study
VIII:518942‐REV (TGTGCCAAGTTGTTAGGTCA)	This study
X:705443‐FWD (TCCCTCTCTGACACCTCTTG)	This study
X:706939‐REV (TGACATGACCCCTTAACTCAGT)	This study
Chemicals, enzymes and other reagents
G418	ThermoFisher	11811023
Ammonium sulfate	Fisher Scientific	BP212R‐1
Dextrose [U‐13C]	Cambridge Isotope Laboratories	CLM‐1396‐5
Dextrose	Sigma‐Aldrich	D9434
Urea	Sigma‐Aldrich	U5378‐1KG
Cycloheximide	Calbiochem	239763‐M
Sodium fluoride	Sigma‐Aldrich	201154
Phenylmethylsulfonyl fluoride	Sigma‐Aldrich	10837091001
Sodium azide	Sigma‐Aldrich	S2002
Tris(hydroxymethyl)aminomethane	Sigma‐Aldrich	252859
Tris base	Roche	TRIS‐RO
Sodium chloride	Sigma‐Aldrich	S7653
Magnesium chloride hexahydrate	USP	1374248
DTT	Sigma‐Aldrich	D0632
Sodium azide	Sigma‐Aldrich	S2002
L‐Leucine	Sigma‐Aldrich	L8000
L‐Isoleucine	Sigma‐Aldrich	I2752
L‐Valine	Sigma‐Aldrich	V0500
Sodium 4‐methyl‐2‐oxovalerate	Sigma‐Aldrich	K0629
3‐Methyl‐2‐oxopentanoic acid sodium salt	Sigma‐Aldrich	198978
Sodium 3‐methyl‐2‐oxobutyrate	Sigma‐Aldrich	198994
Trichloroacetic acid	Sigma‐Aldrich	T6399
Acetone	Sigma‐Aldrich	179124
Protease inhibitor tablets	Roche	4693159001
Phosphatase inhibitor tabets	Roche	4906845001
λ‐phosphatase	New England Biolabs	P0753S
Taq DNA polymerase	New England Biolabs	M0273L
Yeast extract	Sigma‐Aldrich	Y1625
Peptone	Sigma‐Aldrich	P5905
Yeast nitrogen base without amino acids and ammonium sulfate	Fisher Scientific	DF0335‐15‐9
Bacteriological agar	Sigma‐Aldrich	A5306
Glass beads	Scientific Industries	SI‐BG05
Rapamycin	Sigma‐Aldrich	R8781
Software
AccuComp Z2	Beckman Coulter	383550
ImageJ	https://imagej.net/	RRID:SCR_003070
Adobe Photoshop	https://www.adobe.com/products/photoshop.html	RRID:SCR_014199
RStudio	http://www.rstudio.com/	RRID:SCR_000432
SGD	http://www.yeastgenome.org/	RRID:SCR_004694
R	https://www.r‐project.org	v3.6.3 RRID:SCR_001905