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. 2023 Aug 23;10:1201007. doi: 10.3389/fnut.2023.1201007

FIGURE 4.

FIGURE 4

DHM enhances the colocalization and expression of lipophagy-associated proteins in mice exposed to chronic ethanol. DHM administration increases the colocalization and interaction between p62/SQSTM-1 + PLIN-1 + LC3B. (A) Confocal images (upper and lower (magnified) scale bars on images are 10 and 5 μm, respectively) show the expression and colocalization of p62/SQSTM-1 (green), PLIN-1 (red), and LC3B (blue) between groups. (B) Colocalization of p62/SQSTM-1 + LC3B + PLIN-1 as quantified by Pearson’s Correlation Coefficient (PCC) shows a significant increase in colocalization between PLIN-1 + p62/SQSTM-1 (*0.04; brown puncta). (C) Colocalization of p62/SQSTM-1 + LC3B is increased in mice fed EtOH (##0.008; cyan puncta) and highest in EtOH + DHM fed mice (#< 0.0001). (D) Differences in (immunoprecipitated) protein expression levels of Atg7, PLIN-1, and p62/SQSTM-1; (E) LC3B protein expression.