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. Author manuscript; available in PMC: 2023 Sep 6.
Published in final edited form as: Mucosal Immunol. 2023 May 11;16(4):432–445. doi: 10.1016/j.mucimm.2023.05.002

Fig. 6.

Fig. 6

T cell IL-9 promotes antigen-induced mMC-mediated airway reactivity. (A–B) KitWSH/WSH mice were adoptively transferred with HDM-sensitized mMC from WT mice and subsequently treated with HDM for 2 weeks. Following passive sensitization to 2,4-Dinitrophenyl (DNP) and challenge intratracheally with DNP-BSA, airway reactivity was measured for one hour. Experimental design indicated in (A) and results in (B) (n = 5). Data are representative of two independent experiments with similar results. (C) HDM-treated WT mice were intravenously treated with α-IL-9 or isotype control for the last 2 weeks of treatment. Airway resistance was monitored in (C) (n = 4–5). Data are representative of two independent experiments with similar results. (D–E) WT mice transferred with OTII-derived TH2 or TH9 cells were treated with OVA for 6 days. Airway resistance was monitored for 60 minutes in (D) (PBS: n = 7, TH2: n = 10, and TH9: n = 10). Data are pooled from three independent experiments; E, serum MCPT1 expression was assessed via enzyme-linked immunosorbent assay (n = 5); F, airway resistance for HDM-treated Sfpi1fl/flCD4cre and Sfpi1fl/fl mice (n = 5). Data are representative of two independent experiments with similar results. (G–H): G, airway resistance for HDM-treated Il9fl/flCD4cre and Il9fl/fl mice; H, MCPT1 protein expression was determined via enzyme-linked immunosorbent assay (n = 5). Data are representative of two independent experiments with similar results. Error bars indicate ± standard error of mean. Statistical significance was determined by analysis of variance, followed by Tukey’s multiple comparison test (E), Sidak’s multiple comparison test (B, D, F, and G), Student’s t test in (H), and Mann-Whitney U test of area under the curve (B–D, F, and G). ACT = adoptive cell transfer; BALF = bronchoalveolar lavage fluid; CD = clusters of differentiation; HDM = house dust mite; Ig = immunoglobulin; IL = interleukin; MCp = mast cell progenitors; MCPT 1 = mast cell protease 1; mMC = mature mast cells; ns = not significant; OVA = ovalbumin; PBS = phosphate buffered saline; SSC = side scatter; TH = T helper; WT, wild type.