Table 1.
Reagents for flow cytometry, cell culture, and ELISA.
| Flow cytometry antibodies | |||||
|---|---|---|---|---|---|
| Antigen/Name | Clone | Fluorochrome | Company Dilution in FACS buffer | Catalog number | |
| Fixable viability dye | eFluor 780 | eBioscience | 1:1500 | 65–0865-14 | |
| Mouse lineage antibody cocktail: | PerCP-Cy5.5 | BD Biosciences | 1:25 | 561317 | |
| CD3e | 145–2C11 | ||||
| CD11b | M1/70 | ||||
| CD45R/B220 | RA3–6B2 | ||||
| Ly-76 | TER-119 | ||||
| Ly6G and Ly6C | RB6–8C5 | ||||
| Mouse CD45 | 30-F11 | PE/Dazzle 594 | Biolegend | 1:250 | 103145 |
| Mouse CD45.1 | A20 | BV421, BV650 | Biolegend | 1:200 | 110735 |
| Mouse CD45.2 | 104 | BV605, APC | Biolegend | 1:200 | 109841 |
| Mouse CD49b | HMa2 | PE | BioLegend | 1:250 | 103506 |
| Mouse CD49b | Dx5 | BV421 | BD Biosciences | 1:200 | 563063 |
| Mouse c-Kit | 2B8 | FITC, APC | BioLegend | 1:100 | 105805, 105811 |
| Mouse ST2/IL-33R | U29–93 | BV510 | BD Biosciences | 1:100 | 745080 |
| Mouse FceRI | MAR-1 | PE-Cy7 | BioLegend | 1:200 | 134317 |
| Mouse FcyRII/III | 93 | BV510 | BioLegend | 1:200 | 101333 |
| Mouse Integrin β7 | FIB504 | APC, BV711 | BioLegend | 1:200 | 321207, 321239 |
| Mouse CCR2 | 475301 | BV786 | BD Biosciences | 1:200 | 747966 |
| Mouse Ki67 | SolA15 | PE-eFluor610, AF700 | Invitrogen | 1:200 | 61–5698-82, 56–5698-82 |
| Mouse CD3 | 145–2C11 | PerCP-Cy5.5 | BD Biosciences | 1:200 | 551163 |
| Mouse IL-4 | 11B11 | AF647 | BioLegend | 1:200 | 504110 |
| Mouse IL-9 | RM9A4 | PE | BioLegend | 1:200 | 514104 |
| Mouse IL-13 | eBio13A | AF488 | eBioscience | 1:200 | 53–7133-82 |
| Mouse Ly6G | RB6–8C5 | FITC | BD Biosciences | 1:200 | 553126 |
| Mouse SiglecF | E50–2440 | PE | BD Biosciences | 1:200 | 552126 |
| Mouse CD64 | X54–5/7.1 | FITC | BioLegend | 1:200 | 139316 |
| Mouse Mertk | 2B10C42 | PE | BioLegend | 1:200 | 151506 |
| Mouse CD11b | M1/70 | PerCP-Cy5.5 | eBioscience | 1:200 | 45–0112-82 |
| Mouse CD11c | N418 | PE-Cy7 | eBioscience | 1:200 | 25–0114-82 |
| Reagents for T helper cell differentiation | |||||
| Name (Clone) | Company | Catalog Number | |||
| anti-mouse CD3 (145–2C11) | BioXCell | BP-0001–1 | |||
| anti-mouse CD28(37.51) | BioXCell | BE0015–1 | |||
| anti-mouse IFNy (XMG1.2) | BioXCell | BE0055 | |||
| mouse IL-4 (11B11) | BioXCell | BE0045 | |||
| mouse IL-2 (JES6–5H4) | BioXCell | BE0042 | |||
| Human TGF-β1 | Miltenyi Biotec | 130–095-067 | |||
| Reagents for mast cell cultures | |||||
| mouse IL-3 | Biolegend | 575504 | |||
| mouse SCF | Biolegend | 579706 | |||
| ELISA kits | |||||
| Name | Company | Catalog Number | |||
| LEGEND MAX Mouse OVA-specific IgE ELISA | BioLegend | 439807 | |||
| Mouse MCPT1 ELISA | Invitrogen | 88–7503-88 | |||
| Mouse IL-9 ELISA MAX Deluxe | BioLegend | 442704 | |||
CD = clusters of differentiation; IL = interleukin.