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NMR spectroscopy of deuterated cholesterol and ergosterol in model membranes.
2H NMR spectra of ergosterol-d1 (A) and cholesterol- d1 (B) both deuterated in position carbon C3 each 20 mol% incorporated in POPC membranes in the presence (red spectra) or absence (black spectra) of 10 mol% natamycin. Natamycin slows the dynamics of both sterols as inferred from the absence of large quadrupolar splittings (arrows). The measurements were carried out at 25 °C.
