Fig. 7.

Analogs of cholesterol and ergosterol become enriched in the liquid-ordered phase.

Structure of dehydroergosterol (DHE, A) and cholestatrienol (CTL, B), with differences to ergosterol and cholesterol indicated in red. GUVs consisting of POPC/SSM/DHE (1:1:1; C) and of POPC/SSM/CTL (1:1:1; D) with the respective sterol in red and NBD-PC in green, respectively, were examined microscopically. From the microscopic images, the percentage partitioning of DHE and CTL into the Lo phase (lacking NBD-PC, red) or Ld phase (containing NBD-PC, green) was quantified for GUVs with either DHE or CTL (E). See main text for further explanations.