Fig. 2.

IL-33 induced ILC2s, eosinophils, and Tregs in the liver of IRI mice.

(A) Representative FACS analysis showing the gating strategy to identify CD45⁺Lin^-CD127⁺ST2⁺GATA3⁺ ILC2 in mouse liver. (B) Histogram showing expression of CD90, KLRG1, and CD25 on liver ILC2 cells. Specific markers (red lines) and isotype controls (grey-filled areas) are shown. (C and D) Proportion and absolute number of ST2⁺GATA3⁺ ILC2 in the livers of sham, IRI + vehicle, or IRI + IL-33 mice. (E and F) Immunofluorescence staining for CD127 (green), GATA3 (red), and CD3 (magenta) in the liver sections of sham and IRI mice. The white arrowheads indicate ILC2s (CD3^-CD127⁺GATA3⁺). Bar = 100 mm. (G) Proportion of Siglec-F⁺ eosinophils in the CD45+ leucocyte compartment from the liver were measured by flow cytometry. (H and I) Proportion of CD4⁺Foxp3⁺ Tregs in the CD4⁺ T-cell compartment from the liver (H) and liver draining lymph node (I) were measured by flow cytometry. Data shown are the mean ± SEM (n = 4–6 per group). Statistical significance was assessed using a one-way ANOVA. ∗∗∗p <0.001. FSC, forward scatter; hpf, high-power field; ICL2, type 2 innate lymphoid cell; IRI, ischaemia/reperfusion injury; SSC, side scatter; Treg, regulatory T cell.