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. 2023 Aug 2;30(9):2167–2186. doi: 10.1038/s41418-023-01198-x

Fig. 4. GFPT1-induced HBP metabolic acceleration fosters BACH2 O-GlcNAcylation in renal IRI process.

Fig. 4

A, B Schematic delineating the flows and results of O-GlcNAcylation LC-MS/MS analysis of mouse renal tissues (n = 3 group−1). C MS analysis identified residue Thr389 as the BACH2 O-GlcNAcylated site. D sWGA pull-down assays were conducted in mouse renal tissues (n = 6 group−1); IB assays were determined using anti-BACH2. Co-IP assays of BACH2-HA and OGT-Flag were examined using an anti-HA antibody (E) or an anti-Flag antibody (F) in 293 T cells. G Co-IP assays of the endogenous BACH2 and OGT in HK-2 cells.