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. 2023 Aug 8;30(9):2053–2065. doi: 10.1038/s41418-023-01203-3

Fig. 3. Pd1 deficiency results in abnormal neuronal morphology.

Fig. 3

A Representative images of neurons in the upper layer at P7. Control or Pd1-sh1 plasmid was electroporated at E15 and the brains were harvested at P7. B Sholl graphs of dendrites in electroporated neurons at P7 (n = 15 cells from 3 animals). C Representative images of neurons in the upper layer at P14. Brains were electroporated with control or Pd1-sh1 plasmid at E15 and harvested at P14. D Sholl analysis of electroporated neurons at P14 (n = 15 cells from 3 animals). E Graph shows the morphology of neurons cultured in differentiation medium in vitro. GFP plasmid was electroporated into the brains of Pd1cKO mice and Pd1fl/fl mice at E13 to label the NPCs. At E14, GFP expressed NPCs were selected and cultured in differentiation medium for 6 days. F Statistics show that the total dendrites length of neurons is reduced when Pd1 is lost. n = 30 cells and p = 0.003. G Synapsin immunostaining of neurons isolated from WT and cKO mice and cultured in vitro. H The number of puncta per 10 μm dendrite. n = 15 and p = 0.00009. Error bars represent means ± S.E.M.; Two-tailed unpaired t-test, P < 0.05(*), P < 0.01(**), n.s. not significant. The scale bar represents 100 μm (A, C), 20 μm (E), and 10 μm (G).