Table 1.
Key resources for the experiments, including the antibodies, chemicals, primers, kits, animals, software, etc
| Reagent or Resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Anti-δ-secretase (AEP) (11B7) |
Dr. Colin Watts (University of Dundee) |
N/A, 1:1000 for WB, 1:200 for IF |
| Anti-TauN368 | Home-made | N/A, 1:1000 for WB, 1:200 for IF |
| Anti-APPN585 | Home-made | N/A, 1:1000 for WB, 1:200 for IF |
| Anti-C/EBPβ(H-7) HRP | Santa Cruze | Cat# sc-7962 HRP, 1:1000 for WB, 1:200 for IF |
| Anti-phoshpo Thr235 C/EBPβ | Cell Signaling Technology | Cat# 3084 s, 1:1000 for WB, 1:200 for IF |
| Anti-beta Amyloid 4G8 | Covance | Cat# SIG-39220, 1:1000 for WB, 1:200 for IF |
| Anti-Tau (T22) oligomeric | Millipore Sigma | Cat# ABN454, 1:1000 for WB, 1:500 for IF |
| Anti-phospho-Tau (Ser202, Thr205) (AT8) | Thermo-fisher | Cat# MN1020, 1:1000 for WB, 1:200 for IF |
| Anti-Iba1 | VWR | Cat# 100369-764, 1:1000 for WB, 1:200 for IF |
| Anti-CD86 (E2G8P) | Cell Signaling Technology | Cat# 91882 S, 1:1000 for WB, 1:200 for IF |
| Anti-Tau (TAU-5) | Invitrogen | Cat# AHB0042, 1:1000 for WB |
| Anti-PTGES | Thermo-fisher | Cat# PA5-60916, 1:1000 for WB |
| Anti-APP A4 (22C11) | Millipore Sigma | Cat# MAB348, 1:1000 for WB |
| Anti-5 Lipoxygenase (LOX5) | Abcam | Cat# ab39347, 1:1000 for WB |
| Anti-Cyclooxygenase 1 (COX1) | Abcam | Cat# ab109025, 1:1000 for WB |
| Anti-Cyclooxygenase 2 (COX2) | Abcam | Cat# ab15191, 1:1000 for WB |
| Anti-LTB4-R1/BLTR | Thermo-fisher | Cat# BS-2654R, 1:1000 for WB |
| Anti-LTB4-R2 | Thermo-fisher | Cat# BS-2655R, 1:1000 for WB |
| Anti-β actin (AC-15) | Invitrogen | Cat# AM4302, 1:1000 for WB |
| Anti-mouse IgG-HRP | Cell Signaling Technology | Cat# 7076 S, 1:2000 for WB |
| Anti-rabbit IgG-HRP | Cell Signaling Technology | Cat# 7074 S, 1:2000 for WB |
| Anti-mouse IgG-Alexafluor 488 | Invitrogen | Cat# A11001, 1:2000 for IF |
| Anti-mouse IgG-Alexafluor 594 | Invitrogen | Cat# A11005, 1:2000 for IF |
| Anti-rabbit IgG-Alexafluor 488 | Invitrogen | Cat# A11034, 1:2000 for IF |
| Anti-rabbit IgG-Alexafluor 594 | Invitrogen | Cat# A11037, 1:2000 for IF |
| Anti-rabbit IgG-Cy5 | Invitrogen | Cat# A10523. 1:2000 for IF |
| Chemical and antibiotics | ||
| Thioflavin S | Millipore Sigma | Cat# T1892-25G |
| Arachidonic acid | Cayman Chemical | Item No. 90010 |
| Prostaglandin E2 | Cayman Chemical | Item No. 14010 |
| Leukotriene B4 | Cayman Chemical | Item No. 20110 |
| 12-HTTrE | Cayman Chemical | Item No. 34590 |
| Vancomycin Hydrochloride | VWR | Cat# 97062-554 |
| Gentamicin sulfate | Sigma-Aldrich | Cat# G1914-5G |
| Ampicillin | Sigma-Aldrich | Cat# A159325 |
| Erythromycin | Sigma-Aldrich | Cat# PHR1039-1G |
| Neomycin | Fisher Scientific | Cat# 21810031 |
| Critical Commercial Assay | ||
| Amyloid beta 42 mouse ELISA Kit | Invitrogen | Cat# KMB3441 |
| Amyloid beta 40 mouse ELISA Kit | Invitrogen | Cat# KMB3481 |
| Mouse IL-6 ELISA Kit | Abcam | Cat# ab100712 |
| TNF alpha Mouse Uncoated ELISA Kit | Invitrogen | Cat# 88-7324-88 |
| IL-1 beta Mouse Uncoated ELISA Kit | Invitrogen | Cat# 88-7013-22 |
| Prostaglandin E2 (PGE2) ELISA Kit | caymanchemical | Cat# 514010 |
| Leukotriene B4 (LTB4) ELISA Kit | caymanchemical | Cat# 10009292 |
| Deposited Data | ||
| 16 s RNA sequences | PPMS for EIGC https://fex.cores.emory.edu/ | KYE22363-63838 |
| RNAseq data | PPMS for EIGC https://fex.cores.emory.edu/ | KYE22363-64287 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C/EBPβ Tg | Ye lab | N/A |
| Bacteria: Bacteroides fragilis VPI 2553 | ATCC | Cat# 25285 |
| Sequence-Based Reagents | ||
| ALOX5 TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm01182747_m1 |
| PTGS1 TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm00477214_m1 |
| PTGS2 TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm00478374_m1 |
| LTB4R TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm00521839_m1 |
| LTB4R2 TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm00498491_s1 |
| PTGES TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm00452105_m1 |
| GAPDH TaqMan® Gene Expression Assays | Thermo-fisher | Cat# Mm99999915_g1 |
| Software and Algorithms | ||
| ImageJ 1.51J8 | National Institutes of Health | https://imagej.nih.gov/ij/ |
| SPSS 12.0 | IBM | https://www.ibm.com/analytics/spss-statistics-software |
| Prism-GraphPad 7.04 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| QIIME 2 r2023.2 | Dr.Alam Ashfaqul laboratory, University of Kentucky | https://qimme.org/ |
| Illumina’s bcl2fastq | Dr.Alam Ashfaqul laboratory, University of Kentucky | https://www.illumina.com/ |
| PyNAST 1.0 | Dr.Alam Ashfaqul laboratory, University of Kentucky | http://biocore.github.io/pynast/ |