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. 2023 Sep 6;14:5464. doi: 10.1038/s41467-023-41156-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a‒f The cryo-tomographic slices and the 3D segmentation of the minicells. IM: cyan; OM: violet; released material: green. Insets in (a) and (b): enlarged images of the membrane with a pore. The red arrowhead indicates the location of the pore. Lipid clusters are indicated by black arrowheads. The location of blisters and shrunken minicells are indicated by white and yellow arrowheads, respectively. Scale bar = 100 nm. g Counts of pores, minicells with blisters, shrunken minicells, and lipid clusters after the treatment with different concentrations of melittin. h The proportion of the occurrence of pores, minicells with blisters, shrunken minicells, and lipid clusters in 140 tomograms.