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. 2023 Sep 6;14(9):594. doi: 10.1038/s41419-023-06116-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Representative immunofluorescence images from SNCA (A53T)-SY5Y neurons treated with (bottom) or without 20C (Top). Scale bar = 10 µm. B Percentage of infected cells devoid of α-Syn aggregates. C Percentage of infected cells bearing >5 α-Syn aggregates. D Percentage of infected cells of infected SNCA (A53T)-SY5Y containing different α-Syn aggregates. E Representative immunofluorescence images of EGFP-positive cells revealed by imaging flow cytometry. Images of positive cells with 1, 2, 3, 4, >5 α-Syn aggregates are represented from top to bottom. F Percentage of infected cells of infected SNCA (A53T)-H4 containing different α-Syn aggregates. G Percentage of infected positive cells. Error bars are represented as SEM of mean values, n = 3. ***p < 0.001 vs. the control group.