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. 2023 Sep 6;13:14691. doi: 10.1038/s41598-023-41873-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Single particle cryoEM analysis of MBP-FL Vpu soluble oligomers. (A) A micrograph showing representative oligomers and dissociated monomers; (B) representative unsupervised 2-D class averages from 14,657 particles; (C,D) single-particle reconstruction of the two identified particle populations with 11,541 (C) and 2815 (D) respectively. The three images below each 3-D map are the 3 orthogonal projections of the map, making the lower density regions easier to observe. The scale bar is 20 nm.