Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 6;13:14691. doi: 10.1038/s41598-023-41873-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The DEER data and reconstructed inter-spin distances for Vpu spin-labeled at residue N55C. (A) Raw DEER data recorded for two different evolution times, 1 µs (blue) and 3 µs (green). (B) Background subtracted DEER signal for 1 µs evolution time data in (A). The black dashed line indicates the zero-signal amplitude. (C) The reconstructed inter-spin distances for the data in (B). (D) The intense narrow peak in DQC data originates from very short distances on the order of 10 Å for some spin-labels at N55C in Vpu oligomer. These distances are too short to be sensed by DEER whose sensitivity rolls off quickly below 20 Å.