Figure 3.

Western blot validation of selected nuclear-enriched and nuclear-depleted proteins in seizure-induced mice

(A–H) Western blots of nuclear proteins FOS, JUNB, CUL4B, NEDD8, SUMO-1, SUMO-2/3, CaMKIIβ/γ, and NACC1 in whole nuclear lysates from mock- and pilocarpine-injected animals. All proteins bands are reported as mean intensities after normalizing band intensity of interest against LaminB1. Homo, homogenate; Nuc, nuclear extract. N = 6 pairs of animals. All plots were analyzed with paired t-test or Wilcoxon test, and reported as means ± SEM, p < 0.05 (∗).