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. 2023 Aug 15;26(9):107647. doi: 10.1016/j.isci.2023.107647

Figure 2.

Figure 2

MitoQ activated antioxidant system and mitophagy in chondrocytes

Chondrocytes were exposed to IL-1β and MitoQ (0.125,0.25, and 0.5 μM) for 24 h.

(A) The chondrocytes were treated with IL-1β for 1, 6, 12, 24, and 48 h, and then whole-cell lysates together with nuclear lysates were prepared for western blot.

(B, C) The levels of NRF2, HO-1, and NQO1.

(D) Nrf2 expression was examined by qRT-PCR.

(E–G) Nuclear translocation of Nrf2 was observed by immunofluorescence (scale bar: 50 μm) (F and G) The levels of Parkin, PINK1, and LC3 II.

(H) Parkin expression was examined by qRT-PCR.

(I) Chondrocytes were stained with Mitotracker (mitochondria marker, red) and Parkin (green) (scale bar: 25 μm). ∗p < 0.05 vs. the control group, ∗∗p < 0.01 vs. the control group, #p < 0.05 vs. the IL-1β treated group, ##p < 0.01 vs. the IL-1β treated group, Data are represented as mean ± SD.