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. 2023 Aug 15;26(9):107647. doi: 10.1016/j.isci.2023.107647

Figure 6.

Figure 6

Parkin regulated the expression of the NRF2 antioxidant pathway through negative feedback

After Parkin knockdown, chondrocytes were exposed to IL-1β and MitoQ for 24 h.

(A) The level of Parkin.

(B and C) The levels of Parkin and PINK1.

(D and E) The protein levels of iNOS, MMP3, and COL2A1.

(F) The mRNA level of Col2a1.

(G and H) The GPX4 level was measured by western blot and qRT-PCR.

(I and J) The protein levels of NRF2, HO-1, and NQO1, and the mRNA level of Nrf2.

(K and L) The levels of NRF2, HO-1, and NQO1 treated by MitoQ with negative control or si-Parkin.

(M and N) The expression of iNOS, MMP13, and COL2A1. ∗p < 0.05 vs. the IL-1β + negative control group, ∗∗p < 0.01 vs. the IL-1β + negative control group, #p < 0.05 vs. the IL-1β + negative control or IL-1β + MitoQ + negative control group, ##p < 0.01 vs. the IL-1β + negative control or IL-1β + MitoQ + negative control group, Data are represented as mean ± SD.