TABLE 2.

In vitro sperm parameters of milt collected from eight Atlantic salmon (Salmo salar L.) males are presented as mean ± SD for cold-stored and cryopreserved milt. Milt was stored cold for 1 day at 2°C (D1-2°C) and 4 days at 2°C (D4-2°C) and 8°C (D4-8°C), respectively, with subsequent cryopreservation (D1-2°C/Cryo, D4-2°C/Cryo and D4-8°C/Cryo). Different superscripts denote significant differences (p < 0.05) between storage conditions within cold-stored and cryopreserved samples based on a linear mixed model. Asterix denotes significant differences (p < 0.05) between cold-stored and cryopreserved samples.

	Cold-stored			Cryopreserved
	D1-2°C	D4-2°C	D4-8°C	D1-2°C/Cryo	D4-2°C/Cryo	D4-8°C/Cryo
Viability (%)	95.1 ± 0.7a	75.5 ± 8.2b	61.5 ± 12.9c	59.0 ± 7.7a,*	40.6 ± 8.4b,*	29.2 ± 6.6c,*
TM (%)	35.8 ± 11.5a	6.6 ± 4.9b	1.5 ± 2.1b	32.7 ± 9.1a	8.5 ± 4.8 b	3.6 ± 2.6b
PM (%)	32.4 ± 11.4a	4.7 ± 3.6b	0.7 ± 1.3 b	29.7 ± 9.3a	7.2 ± 4.5b	2.5 ± 2.3b
VAP (µm/s)	86.1 ± 7.8a	56.9 ± 6.4b	27.4 ± 12.5c	97.5 ± 10.1a,*	72.3 ± 10.5b,*	53.7 ± 14.0c,*
VCL (µm/s)	100.6 ± 8.9a	71.1 ± 6.5b	41.3 ± 12.7c	107.7 ± 9.7a	83.6 ± 9.5b,*	65.5 ± 14.9c,*
VSL (µm/s)	59.9 ± 8.7a	42.2 ± 6.8b	15.3 ± 11.8c	85.3 ± 9.5a,*	61.1 ± 10.6b,*	40.6 ± 11.6c,*
STR (%)	69.1 ± 7.1a	71.1 ± 5.3a	45.7 ± 16.2 b	85.3 ± 2.3a,*	79.2 ± 6.1a,*	69.8 ± 8.8b,*
LIN (%)	59.2 ± 6.3a	55.5 ± 6.0a	32.3 ± 14.2b,*	75.5 ± 3.1a,*	66.0 ± 9.4b,*	56.5 ± 7.2c,*
ATP (nM)	428.5 ± 41.8a	132.8 ± 43.3b	19.7 ± 37.7c	436.9 ± 45.7a	154.9 ± 70.2b	37.6 ± 25.0c
DFI (%)	0.8 ± 0.1a	1.7 ± 0.7b	3.3 ± 0.5c	2.3 ± 0.5a,*	3.5 ± 0.6b,*	4.3 ± 1.0c,*
HDS (%)	1.9 ± 0.3a	5.0 ± 2.0b	10.0 ± 1.6c	2.8 ± 0.7a	5.7 ± 1.1b	7.0 ± 1.3c,*

TM, total motility; PM, progressive motility; VAP = velocity average path; VCL, velocity curvilinear; VSL, velocity straight-line; STR, straightness; LIN, linearity, DFI = DNA, fragmentation index; HDS, High DNA, stainability.