Fig. 4. Confocal images of CA_0.1% TPP (a, c, e and g) and CA_1.0% TPP (b, d, f and h) obtained using 20× objective; (a and c) spectral image and spectra of ROI for excitation at 405 nm; (b and d) spectral image and spectra of ROI for excitation at 488 nm; (e–h) FLIM images for excitation at 640 nm and emission collected at 715 nm; (e and g) images showing the average fluorescence lifetime with color scale covering the 6–10 ns range; (f and h) images showing the intensity I1 and I2 for the long (red) and short (green) lifetime, respectively.
